Using
polymerase chain reaction (
PCR), a portion of the
cytochrome oxidase 1 (446
nucleotide and 148
amino acid) of
mitochondrial genome of
adult worm of small
liver fluke from
human in Hiep Hoa district, Bac Giang (CsNgBG) province was amplified. The
nucleotide and
amino acid of these sequences were comparatively analyzed with the known
Clonorchis sinensis. The
analysis revealed that the Bac Giang Clonorchis sp (in
human) was
similar (100%
nucleotide and
amino acid) to the Nam Dinh
Clonorchis sinensis (identification was done); 99.6% (
nucleotide) and 100% (
amino acid) homology to the C.sinensis of
Chinese strain (CsCN-Gen Bank AF 184619) and Korean
strain (CsKor-Gen Bank AF 181889). Thus, the Bac Giang Clonorchis sp is identified as
Clonorchis sinensis in
Opisthorchidae family, Clonorchis genus.
Phylogenetic analysis uniquely placed the Bac Giang
Clonorchis sinensis to the group of
Chinese and Korean
Clonorchis sinensis.