Objective To study the expressions of
IL-1 receptor type Ⅰ(IL-1RI)
mRNA and
IL-1?
protein in the
rat carotid body.
Methods In situ hybridization,
immunofluorescence double
staining and
Western blotting methods were used. Results The result of
in situ hybridization showed that the positive signal of
IL-1?
mRNA was mainly located in the glomus
cells of the
carotid body.The result of
immunofluorescence double
staining showed that
IL-1?
protein also expressed in the glomus
cells of the organ.The
Western blotting proved that the
IL-1? immunoreactive band appeared at 18kD,consistent with the
molecular weight of the
cytokine.Conclusion The glomus
cells of the
rat carotid body not only express IL-1RI
mRNA,but also
IL-1?.