After murine fetal
liver cells (FLC) were transfected with
granulocyte-macrophage colony-stimulating factor (
GM-CSF )
gene by recombinant
adenovirus and intrasplenically transplanted into allogeneic
mice , the effects of
GM-CSF gene -transfected FLC on the recovery of
immune response inhibited by
chemotherapy were observed. The number of CD4 +
cells and the ratio of CD4 + /CDS +
cells from peripheral
blood lymphocytes increased significantly. The cytotoxicity of the
NK cells and the proliferation response of splenocytes to ConA, LPS elevated markedly, but the same results were not from
bone marrow . These data demonstrated that intrasplenic
transplantation of
GM-CSF gene -transfected FLC could effectively accelerate the recovery of
immune response after high-
dose chemotherapy .