Objective To investigate the inhibitory effect of
celecoxib on
non-small cell lung cancer in vitro and the
role of
survivin.
Methods The inhibitory effect of
celecoxib was detected by MTT.
Flow cytometry and
electron microscope were used for evaluation of
apoptosis.The expression of
survivin was detected by RT-PCR and
Western blot.Results ①
Celecoxib inhibited
cell survives in a
time-dependent and concentration-depended manner,and the effect of
celecoxib was more significant in NCI-H520 than in A549.②
Celecoxib caused
apoptosis in concentration-depended manner.3.
Celecoxib decreased the expression of
survivin.Conclusion The decrease of the expression of
survivin may
play a critical
role in the
apoptosis of NSLLC
in vitro induced by
celecoxib.