Mobilization of peripheral blood stem cells in mice with short-duration and high-dose recombinant human granulocyte colony-stimulating factor / 中国组织工程研究
To modify the common PBSC mobilization, and investigate mobilization effect of PBSC in KM mice with short-duration and high-dose rhG-CSF. DESIGN, TIME AND
SETTING:
A randomized controlled animal trial was carried out in the laboratory of Hematology, the Second Affiliated Hospital to Dalian Medical University from September 2006 to March 2007. MATERIALS Twenty-four KM malemice of 8 months old were divided into 3 groups according to the administration conventional regimen group, short-duration and high-dose group, and saline control group.
METHODS:
Mice in the conventional regimen group were injected subcutaneously with rhG-CSF (0.2 ?g one time, twice a day for 6 days). Mice in the short-duration and high-dose group were injected with equal volume of normal saline at first 4 days, and then with rhG-CSF (2.5 ?g one time, twice a day for 2 days). Mice in the saline control group were injected subcutaneously with equal volume of normal saline for 6 days. MAIN OUTCOME
WBC in the peripheral blood of KM mice increased rapidly in conventional regimen group and short-duration and high-dose group. No obvious change of WBC was observed in the control group. The number of colony-forming unit-granulocytemacrophage in conventional regimen group and short-duration and high-dose group was significantly elevated over 50 folds than control group (P 0.05).
CONCLUSION:
There is a good efficacy and feasibility in PBSC mobilization of KM mice with short-duration and large-dose rhG-CSF, and it is a successful amelioration of PBSC mobilization of mice peripheral blood.