Objective To compare the inducing effect of three kinds of
connective tissue from
amnion,fetal
mesentery and intestinal submucosa on the differentiation of fetal BMSCs
in vitro.
Methods The
epithelium on three kinds of
connective tissue were sampled and
EGF and
IGF-1 in these
tissue clumpes were detected.DAPI-P3-BMSCs were planted on the surface of three kinds of
connective tissue to
culture.There were
connective tissue induced groups,
connective tissue and
EGF and
IGF-1 induced groups,
EGF and
IGF-1 induced as
control group.The
tissue clump was collected at 8 d、10 d、12 d,to compare their inducing effect on the differentiation of BMSCs.Results All of BMSCs induced by three kinds of
connective tissue and
growth factors expressed CK or EMA.The effect of unite-induced groups was stronger than those groups induced by only
connective tissue,P