Objective:
To establish the model of
rats with UC by 2,4,6 trinitrobenzene
sulfonic acid ( TNBS)/
ethanol to understand the changes of
IL-17,
IL-23,TGF-β1,IFN-γin pathogenesis of
ulcerative colitis (UC) and the impacts of
mesalazine,mont-morillonite
powder and
clostridium on that.
Methods:
85
rats were divided randomly into 6 groups including the model ,
mesalazine,
montmorillonite,
clostridium,
mesalazine combined with
montmorillonite powder group (15
rats per group ) and an additional
control group of 10 normal
rats.TNBS/
ethanol was used to establish the model of
rats with UC.The
dosage of model,
mesalazine,
montmorillonite powder ,
clostridium and
mesalazine combined with
montmorillonite powder were converted by surface area of
rat and given to the
rats by gastric-tube daily.All
rats were executed at the 12th day of the molding and
treatment ,and the
blood and
colon samples were collected.The content of
IL-17,
IL-23,TGF-β1,IFN-γwas measured in the
blood by the
ELISA method.The different changes of the four
cytokines were compared separately.
Results:
( 1 ) TNBS/
ethanol was used to establish the model of
rats with UC.The obvious
inflammation can be observed on distal
colon of
rats by pathologic sections of HE stained .(2) The levels of
plasma IL-17,
IL-23 and IFN-γin the model,
mesalazine,
montmorillonite,
clostridium and
mesalazine combined with
montmorillonite powder group were higher than ones in the control gruop (P<0.05),meanwhile,the levels of
plasma TGF-β1 are lower (P<0.05).3.Comparing with the model group,the levels of
plasma IL-17,
IL-23 and IFN-γin the
mesalazine,
montmorillonite,
clostridium and
mesalazine combined with
montmorillonite powder group were lower , and the levels of TGF-β1 were higer.The differences have
statistic significative ( P<0.05).(4)Comparing the
efficacy of
treatment among
mesalazine ,
montmorillonite powder,
clostridium and
mesalazine combined with
montmorillonite powder ,
mesalazine and
mesalazine combined with
montmorillonite powder gets the best results ( P<0.05 ).The
efficacy between
montmorillonite powder and
clostridium is
similar.
Conclusion:
(1) TNBS/
ethanol can be used to establish the model of
rats with UC and can be successfully approved by the measurement of symptoms and pathologic investigation .(2)The
IL-17,
IL-23 and IFN-γare very active higher in the
blood of
rats with experimental
ulcerative colitis induced by TNBS /
ethanol, and have a positive correlation with
inflammation.The TGF-β1 is in decrease in the
blood of
rats with
colitis , and has a Negative Correlation with
inflammation.(3)
Mesalazine,
montmorillonite powder,
clostridium and
mesalazine combined with
montmorillonite powder may renovate the damage of inflammatory
tissues of
rats with experimental
ulcerative colitis , and relieve the symptom of
inflammation by reducing exudation of proinflammatory
cytokines IL-17,
IL-23 and immune regulatory
cytokines IFN-γby producing the exudation of proinflammatory
cytokines TGF-β1 to be good for recovery of UC.