Simultaneous Determination of Baicalin,Baicalein and Wogonin in Huatan Pingchuan Tablet by HPLC / 中国药房
Xiang CHEN; Hongping ZHANG; Jingjing XU.
China Pharmacy
; (12): 846-847,848, 2016.
Artículo
en Zh
| WPRIM | ID: wpr-605641
OBJECTIVE:
To establish a method for the simultaneous determination of baicalin,baicalein and wogonin in Hua-tan pingchuan tablet.METHODS:
HPLC was performed on the column was Diamonsil C18 with mobile phase of methanol-0.1%phosphoric acid(gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 277 nm,column temperature was 30 ℃, and the injection volume was 10 μl.RESULTS:
The linear range was 0.069 04-0.690 4 μg for baicalin(r=0.999 4),0.054 56-0.5456 μg for baicalein(r=0.999 6)and 0.030 36-0.303 6 μg for wogonin(r=0.999 1);RSDs of precision,stability and reproduc-ibility tests were lower than 2%;recoveries were 97.64%-99.06%(RSD=0.57%,n=6),98.31%-100.64%(RSD=0.85%,n=6) and 97.53%-99.48%(RSD=0.76%,n=6).CONCLUSIONS:
The method is simple,stable and reproducible,and can be used for the contents determination of baicalin,baicalein and wogonin in Huatan pingchuan tablet.
Biblioteca responsable:
WPRO
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