Spoligotyping technique: improvement and application in classification Mycobacterium tuberculosis

Lien Thi Kim Vu; Au Thi Hai Tran; Khanh Van Tran; Nga Thi Quynh Do; Hung Van Nguyen; Dung Thi Thu Nguyen; Trao Tan Vu; Ulf R.Dalhe

Background:

There are many methods used in epidemiological studies of tuberculosis (TB) bacteria but Spoligotyping method is widely used with high accuracy, simple procedure, and carried out on strains containing a little of IS6110 segment \r\n', u'

Objectives:

To improve Spoligotyping technique and apply this technique to classify Mycobacterium tuberculosis\r\n', u'Subjects and

method:

Subjects and

methods:

The study included 12 medical waste samples collected at Thai Binh Hospital of Tuberculosis and 19 samples obtained from the The Hanoi Institute for Tuberculosis and Lung Diseases. Spoligo model of 31 samples were analyzed based on Spotclust and SpoIDB4 database and divided up into family and subfamily.\r\n', u'

Results:

Spoligotyping technique has good results with the PCR product amplified 40 cycles and presented the film in 18 hours. Obtained results in 31 medical waste samples belong to 4 families Beijing, EAI, T1 and H3-LAM9. EAI and Beijing are dominant families with 45.16% and 38.7%), respectively. T1 and H3-LAM9 are 12.9% and 3.22%, respectively. The number of samples in the study is little but the obtained rate of different spoligo models of strains are quite diversified (41.9%)\r\n', u'

Conclusion:

This result is relatively appropriate with previous studies on the distribution of EAI and Beijing families in Vietnam and the world. Spligotyping technique distinguished samples belonging to Beijing or non-Beijing families, which support for clinical treatment and development of new vaccines.\r\n', u'\r\n', u'\r\n', u'

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