Objective · To globally study the influence of arsenite to the various
biological pathways of
Escherichia coli as a model organism.
Methods · The
protein-arsenite interactions was globally studied based on a
proteome microarray constructed by 4256 affinity-purified
Escherichia coli proteins. The functions of interacting
proteins and their network were then analyzed by
bioinformatics. Results · 91
proteins that remarkably interact with
arsenic were successfully identified.
Bioinformatics analysis found that most of the
proteins possess catalytic activityand are involved in various
biosynthesis and cellular
metabolism pathways. The interactions of
arsenic with
proteins encoded by malY, cfa and hypF
genes were further validated by
Western blotting, which proves the results of
proteome microarray reliable. Conclusion · Arsenite interacts with a variety of
enzymes ofEscherichia
coli and can greatly
affect its
biological metabolism.