Objective To explore the protective
role and mechanisms of
glucagon-like peptide-1(
GLP-1) on
advanced oxidation protein products (
AOPPs)-induced
apoptosis of
cardiomyocytes.
Methods The H9C2
cells were selected in this study and divided into blank
control group,RSA
control group,and groups treated with indicated concentrations of
AOPPs with or without
GLP-1,and
AOPPs +
GLP-1+LY294002 for 24 hours respectively.
Cell viability was detected by
CCK-8 assay. The ROS level was detected by DCFH-DA
fluorescent probe. The
cell apoptosis was tested by fluorescent
staining and
flow cytometry. The expression of p-Akt,p-Bad,Bcl-2,Bax,and active-
caspase-3 proteins were evaluated by
Western blot. Results
GLP-1 attenuated
AOPPs-induced cytotoxicity[(0.929±0.083) vs (1.409±0.099),P<0.01],decreased
AOPPs-induced ROS[(47.817±0.878)% vs (25.413±2.597)%,P<0.01] and
apoptosis[(15.773±3.130)% vs (9.715±0.757)%,P<0.01].
GLP-1 improved
AOPPs-induced
phosphorylation of Akt and Bad,increased the expression of Bcl-2,and decreased the expression of Bax and the activation of
caspase-3. Conclusion
GLP-1 protects
cardiomyocytes against
AOPP-induced
apoptosis,predominantly via the PI3K/Akt/Bad pathway.