Objective To evaluate the effect of silencing ACAT1
gene on
colon cancer cells proliferation,migration,invasion and
colon cancer development by using the small interference
RNA (
siRNA) in
colon cancer cell line HT-29.
Methods Acyl coenzyme A cholesterol acyltransferase 1 (ACAT1)
gene was silenced in
HT-29 cell lines using Hiperfect
transfection reagent.The expression level of ACAT1 was detected by
real time PCR.CFSE and transwell assays were used to evaluate the effect of ACAT1
gene interfering on
cells proliferation,mi gration and invasion.Result ACAT1
mRNA expression decreased obviously after
siRNA interference.Compared with pre-
transfection,proliferation,migration and invasion of
colon cancer cells have been significantly inhibited (P < 0.05).Conclusion ACAT1
gene interference reduced proliferation,migration and of invasion of
HT29 cells,which provide a new potential target for
colon cancer treatment.