Objective To detect
serum antinuclear antibody (ANA) with
enzyme-linked
immunosorbent (
ELISA) and linear immunoblot
technique (LIA) and to evaluate and compare the performance of two
methods in auxiliary
diagnosis of
systemic lupus erythematosus (SLE).
Methods ANA detected by both
ELISA and LIA of 597 cases were collected in the last two years. The results were retrospective analyzed. The
sensitivity,
specificity, accuracy,
positive predictive value,
negative predictive value, and the consistency of the two
methods were compared and analyzed. Results The
sensitivity of
ELISA and LIA was 79.3% and 86.6% respectively for SLE
patients;
specificity 81.7% and 77.3%; accuracy 81.4% and 78.6%,
positive predictive value 40.9%, and 37.8%;
negative predictive value 96.1% and 97.3% respectively. No significant difference was found between the two
methods (P > 0.05). The results showed that the coincidence rate was 81.4%, and the Kappa test 0.55. The Kappa test of the two
methods in SLE group was 0.403. Conclusion No statistical significance is found in the comparison of
ELISA method and LIA
method to detect ANA for the
diagnosis of SLE and the combination of two kinds of
detection method has important application value.