The aim of the study is to determine the neuronal and glial
gene expression of cultured
human amniotic
epithelial cells (HAECs) in
serial passages. HAECs obtained from
human term placentae were cultured in F12DMEM (11) + 10% FBS +10ng/ml
EGF in
serial passages. Quantitative RT-PCR was used to assess the
gene expression analysis. The results showed that the cultured HAECs expressed the
neural stem cell genes (
Nestin, NSE and
Vimentin), mature neuronal
genes (TH, MAP-2, beta-III-
tubulin and NFM) and glial
genes (
CNPase, MBP and Olig). These
neural stem cell genes increased with
serial passages while the
genes expression for mature neuronal and
glial cells were downregulated. These results suggested that HAECs may promote or involve in
neurogenesis and gliagenesis.