Objective To explore the
association of
Toll-like receptor 4 TLR4 and
lipopolysaccharide receptor CD14
gene polymorphisms with
Kawasaki disease (KD) susceptibility.
Methods Three-
color fluorescent
staining flow-cytometry was used to detect the expression of TLR4 in peripheral
blood white blood cell of 76 KD
children and 118 healthy
control group.The
gene of TLR4 (-896A/G), (-1196C/T) and CD14 (-260C/T) polymorphisms was identified by
polymerase chain reaction-
restriction fragment length polymorphisms; and the relationship between
genotype and KD was analyzed.Results 1.The values of mean
fluorescence intensity (MFI) of TLR4 in peripheral
blood white blood cell of the KD groups and the healthy
control groups were 2.87?0.96, 10.55?4.87, 23.36?8.28 and 3.26?0.65, 7.55?1.21, 25.41?6.97, respectively; There was a gradual increase of these values on
lymphocyte, neutrophilic
leukocyte and mononuclear
cell in both groups.2.(-896A/G), (-1196C/T) polymorphisms of TLR4
gene were not found in both groups.3.The frequency of each
genotype of CD14
gene (-260C/T) was 35.5%CC, 30.3%CT, 34.2%TT in KD group and 38.1%CC, 47.5%CT, 14.4%TT in healthy
control group.The frequency of each
genotype was significantly different in 2 groups(?2=11.62 P