Objective To
analysis the
etiology and molecular
classification of
brucella strains isolated in Guangdong province in 2010.
Methods The
strains of 19
brucella were verified and identified by some
methods including traditional
biology phenotype confirmation,
PCR amplification and
pulsed field gel electrophoresis (PFGE).Results On
phenotype level,4
strains were
brucella melitensis biovar 1,2
strains were
brucella suis biovar 3,and the
rest were
brucella melitensis biovar 3,which were specific B
genes positive
strains,and the PFGE typing
similar values ranging from 67.9% to 100%.In addition to the four
strains from Zhuhai for the outbreak,the homology was 100%,and the
rest were sporadic cases.Conclusions
Brucella cases,in Guangdong province,are highly sporadic and dispersed
outbreaks.Compared with a few years ago,it shows species diversification,and
brucella melitensis biovar 3 is still the dominant
serotype.PFGE can be used to distinguish the three species of
brucella,but it can't effectively distinguish the allotypes.