Since 2002,
adult worms of
human small
liver fluke in some provinces were identified by morphology and confirmed by molecular
method as
Clonorchis sinensis in Thanh Hoa, Ninh Binh, Nam Dinh and Bac Giang province;
Opisthorchis viverrini in Phu Yen and Binh Dinh province. This study, using
polymerase chain reaction (
PCR), a portion of the
cytochrome oxidase 1 (410
nucleotide and 136
amino acid) of
mitochondrial genome of
adult worms of small
liver fluke from
human in Nghe An (CsNA) and Dong Nai (CsDN) province were amplified. The
nucleotide and
amino acid of these sequences was comparatively analyzed with the known
Clonorchis sinensis (Nam Dinh
strain). The
analysis revealed that the Nghe An Clonorchis sp and Dong Nai Clonorchis sp (in
human) were homology (99.5 - 99.8%
nucleotide) and
similar (100%
amino acid) to the Nam Dinh
Clonorchis sinensis (identification was done). Thus, the Nghe An and Dong Nai Clonorchis sp were identified as
Clonorchis sinensis in Opisthorchiidae
family, Clonorchis genus.