Wound healing is composed of a complex process that requires harmonies of various
cell populations where
fibroblasts play the main
role. Oligomeric
procyanidins (OPC) are main components of
grape (
Vitis vinifera)
seed extracts, and recent studies showed OPC's effects on
inflammation,
cell migration, and proliferation. We investigated the effect of OPC on
fibroblasts to regulate
wound healing process.
Human dermal
fibroblast known as Hs27
cells were treated with various concentrations of OPC (0, 2.5, 5, 10, and 20 µg/µl).
Cell cytotoxicity was evaluated by the
Cell Counting Kit assay, and the expression levels of secreted
procollagen were analyzed.
Procollagen levels in OPC treated
cells exposed to
transforming growth factor beta 1 (TGF-β1) or
ascorbic acid were evaluated using
Western blot and
immunocytochemistry. Relative
mRNA expressions of
procollagen,
molecular chaperone such as HSP47, P4H were determined by
real-time PCR in OPC treated
cells. OPC showed no cytotoxicity on Hs27
cells at every concentration but inhibited
procollagen secretion in a
dose-dependent manner. The inhibitory effect also appeared under TGF-β1 induced
collagen overproduction.
Immunocytochemistry showed that higher levels of intracytoplasmic
procollagen were accumulated in TGF-β1
treatment group, whereas
ascorbic acid induced a release of accumulated
procollagen under OPC
treatment. The
mRNA expressions of
procollagen,
molecular chaperone were not affected by OPC, but
procollagen level was increased when exposed to TGF-β1. OPC inhibits
procollagen secretion from
fibroblasts with no effects on
cell proliferations even under the
environment of TGF-b1-induced
collagen overproduction. OPC could regulate the
diseases and symptoms of abnormal overabundant
collagen production.