Effect of genistein on ammonia-induced NF-κB activation in astrocytes / 中国病理生理杂志
Dan YANG; zhi Gui JIA; hui Ming CHU; liang Hong DAI.
Chinese Journal of Pathophysiology
; (12): 1598-1601, 2017.
Artículo
en Zh
| WPRIM | ID: wpr-660651
AIM:
To explore the effect of genistein on ammonia-induced nuclear factor-κB (NF-κB) activation and the underlying mechanism.METHODS:
Primary astrocyte cultures were prepared and challenged with NH4Cl to establish a hyperammonemic model.The activation of ERK,Akt and NF-κB was examined by Western blot.RESULTS:
AG1478 and genistein significantly inhibited ammonia-induced activation of ERK and Akt.Ammonia-induced NF-κB nuclear translocation was significantly inhibited by the pretreatment of LY294002,genistein and AG1478.CONCLUSION:
Genistein significantly inhibited ammonia-induced ERK activation and Akt-mediated NF-κB activation,which might represent the important mechanism by which this naturally occurring substance exerts its swelling-inhibiting effect.
Biblioteca responsable:
WPRO
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