Objective To observe the
time dependent changes of
pancreas and
lung injury in
mice acute pancrea-titis induced by
caerulein,and compare the effects of
mice acute pancreatitis model induced by different
injection protocols of
caerulein and
lipopolysaccharides.
Methods Acute pancreatitis were induced by different
injection frequency of
caerulein or
caerulein combined with
lipopolysaccharides, and
plasma amylase activity was detected by
starch-
iodine colorimetry,and
pancreas and
lung injury severity was observed on
paraffin section after HE stai-ning. Results The
injury of
pancreas and
lung in
mice acute pancreatitis induced by
caerulein was most severe at 4 h, which began to repair at 24 h, and basically returned to normal at 5 d. 4 h after last
injection, the
amylase activity in CAE7 group and CAE9 group was higher than that in the
control group(6 461±1 078 U/dl vs 3 093± 331 U/dl;6 821 ± 495 U/dl vs 3 093 ± 331 U/dl, all P<0.001), and CAE7+LPS group was higher than in CAE9 group[(8 912±465)U/dl vs (6 821±495)U/dl,P<0.001],the histological score in CAE7 group and CAE9 group was higher than that in the
control group(4.750 ± 0.524 vs 0 ± 0; 4.917 ± 0.664 vs 0 ± 0, all P<0.001), and that in CAE7+LPS group was higher than in CAE9 group(7.167 ± 0.258 vs 4.917 ± 0.664, P<0.001). Conclusions
Mice acute pancreatitis can be successfully induced by 7 i.p.
injections of
caerulein (50 μg/kg
body weight) at 1-hour intervals,
caerulein combined with
lipopolysaccharides.