Inhibitory role of BANK in progression of experimental autoimmune encephalo-myelitis / 中国免疫学杂志
Hua Gui JIN; Hua Hong LI; Xue LI; Manabu FUJIMOTO; Gao Qing ZHANG.
Chinese Journal of Immunology
; (12): 1854-1857,1861, 2017.
Artículo
en Zh
| WPRIM | ID: wpr-663685
Objective:
To investigate the role of B cell adoptor protein with ankyrin repeats ( BANK ) in experimental autoimmune encephalomyelitis ( EAE) .Methods:
C57BL/6 mice and BANK-deficient ( BANK-/-) mice were immunized with MOG peptide in CFA,and then observed the clinical symptoms and pathological severity .Results:
The percentages of CD4+T cells,CD8+T cells and regulatory T cells in brain and spleen were analyzed by flow cytometry .BANK-/-mice showed significantly higher score at the peak and the plateau phase compared with wild-type mice(P<0.05).HE staining showed more widespread areas of inflammation and demyelination in BANK-/-mice when compared to wild-type mice on day 16.In addition,the frequency of CNS-infiltrating CD8+T cells was markedly higher in BANK-/-mice than in wild-type mice.In addition,the percentage of CD8+T cells from spleen in BANK-/-mice was also increased compared with wild-type mice (P<0.05).By contrast,the percentage of regulatory T cells and the ratio of CD 4/CD8 T cells from spleen in BANK-/-mice were significantly lower than in wild-type mice(P<0.05).Conclusion:
Thus,BANK expression in B cells can inhibit the development of EAE .
Biblioteca responsable:
WPRO
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