Objective To investigate the effect and mechanism of Taohe Chengqi Decoction(TCD)on intestinal function of severe
acute pancreatitis (SAP)
rats.
Methods Fifty SD
rats were randomly divided into blank
control group, SAP model group, TCD group (in the
dosage of 23.5 mL·kg -1·d -1 by gastric gavage),
Octreotide group(in the
dosage of 10 μg·kg-1·d-1 by
intraperitoneal injection),combined
treatment group(TCD in the
dosage of 23.5 mL·kg -1·d -1 by gastric gavage,
Octreotide in the
dosage of 10 μg·kg -1·d -1 by
intraperitoneal injection). Except for the blank
control group,the
rats in the other groups were injected with
L-arginine intraperitoneally to establish SAP model. The
rats were sacrificed 24 h after the model was successfully established. The
serum level of
amylase(AMY) in
rats was detected by biochemical
method. The
serum levels of
interleukin (IL)-6,
IL-10 and
tumor necrosis factor (TNF)-α in the fecal
flora of
rats were measured by
enzyme-linked immunosorbent assay (
ELISA),and the pathological changes of colorectal
tissues and
pancreas tissues were observed by
hematoxylin-
eosin (HE)
staining. Results Compared with the blank
control group,the levels of
serum AMY,TNF-α,
IL-6 and
IL-10 in SAP model group were significantly increased (P < 0.05),
enterococcus and
enterobacteria count were increased, while
lactobacillus and bifidobacteria count were decreased;the histological structure of
pancreas tissues and large intestinal
tissues was destroyed, and the proliferation of large amount of inflammatory
cells was present. Compared with the SAP model group,
serum AMY,TNF-α,and
IL-6 levels were decreased and
IL-10 level was increased in the three medication groups (P<0.05);
enterococcus and
enterobacteria count were decreased,while
lactobacillus and bifidobacteria count were increased;the pathological features of
pancreas tissues and large intestinal
tissues were improved;the effect of the combined
treatment group was the strongest. Conclusion TCD can improve the intestinal function and
intestinal flora of SAP
rats,reduce the inflammatory reaction,and prevent SAP from deterioration.