Objective To investigate the relationship between spatial distribution of default mode network and glucose uptake.Methods Nine healthy subjects were scanned with hybridPET/MR.Resting state MRI (rs-fMRI) and PET data were obtained.Spatial distribution analysis was performed between default mode network and glucose uptake.The relationship between the functional connectivity of default mode network and the distribution of glucose uptake were further analyzed.Results The similar spatial distribution pattern was found between default mode network and glucose uptake.Correlation between functional connectivity and glucose uptake in the default mode network showed that the best correlation coefficient between the values of functional connectivity and relative glucose uptake (rGU) was achieved in the right posterior cingulate cortex (rs =0.833,P<0.001).Conclusion HybridPET/MR is very important to investigate neural mechanism of default mode network.