Objective:
To prepare various
crude extracts using different polarities of
solvent and to quantitatively evaluate their total
phenol,
flavonoids contents and
phytochemical screening of
Thymus vulgaris collected from Al Jabal Al Akhdar, Nizwa, Sultanate of
Oman.
Methods:
The leave sample was extracted with
methanol and evaporated. Then it was defatted with
water and extracted with different polarities organic
solvents with increasing polarities. The prepare
hexane,
chloroform, ethyl
acetate,
butanol and
methanol crude extracts were used for their evaluation of total
phenol,
flavonoids contents and
phytochemical screening study. The established conventional
methods were used for quantitative
determination of total
phenol,
flavonoids contents and
phytochemical screening.
Results:
Phytochemical screening for various
crude extracts were tested and shown positive result for
flavonoids,
saponins and
steroids compounds. The result for total
phenol content was the highest in
butanol and the lowest in
methanol crude extract whereas the total
flavonoids contents was the highest in
methanol and the lowest
hexane crude extract.
Conclusions:
The
crude extracts from locally grown
Thymus vulgaris showed high concentration of
flavonoids and it could be used as
antibiotics for different curable and uncurable
diseases.