Objective To observe the adipocytic differentiation potential of
bone marrow stromal cells (BMS), and the effect of
simvastatin on adipocytic differentiation of
bone marrow stromal cells in vitro, and to elucidate the mechanisms of
anabolic effect of
simvastatin on
bone formation.
Methods BMS from
femur and
tibia of
adult female BALB C mice were cultured
in vitro. Changes of
alkaline phosphatase (ALP) activity were determined
after treatment with adipogenetic agonist (
hydrocortisone 0 5 ?mol/L and
indomethacin 60 ?mol/L, HI) for 6 days. Thenexpression of
lipoprotein lipase (LPL)
mRNA was detected by RT
PCR after treatment with HI and different concentration of
simvastatin for 72 h. Adipogenetic differentiation were also observed with Oil Red O
staining and
fluorescence activated cell sorting (FACS)
after treatment with HI and different concentration of
simvastatin or 100 ?g/L rhBMP 2 for 12 days. Results After BMS were treated with HI for 6 days, ALP activity was significantly decreased ( P