Background: Heparansulfate Interacting
Protein (
HIP ) is a
protein that belongs to a novel class of
heparin and heparansulfate
binding protein . It
plays an important
role in
extracellular matrix structure and function,
cell -
cell and
cell -
extracellular matrix adhesion,
growth and differentiation.
HIP was shown to be expressed in normal epithelia and
epithelial cell lines at both
mRNA and
protein levels. Especially,
HIP was found to be up-regulated in some
cancer cell lines and related to different status and
metastasis .\r\n', u'
Objectives: To determinate
HIP transcript level of
mRNA in
breast cancer tissues in comparison with normal
tissues ; to compare
HIP transcript level at different
cancer stages and
cancer cell types. \r\n', u'Subjects and
method: Total
RNA was isolated from 62
tissue samples (47
breast cancer and 15 normal
tissues );
cDNA synthesis by reverse transcript \u2013polymerase
chain reaction (RT \u2013
PCR );
determination of
HIP transcript using semi-quantitative RT \u2013
PCR . \r\n', u'
Results: HIP transcript was particularly up \u2013 regulated in
breast cancer tissues compared to normal
tissues , especially this up-regulated in
cancer tissues at different stages of development and
cancer cell types. \r\n', u'
Conclusion: These results show that the
HIP transcript level was different between
breast cancer and normal
tissues and its expression was related to different status and
metastasis in
human cancer cell lines .
HIP may be used as a prognostic marker for
breast cancer .\r\n', u'