This study aims to understand the
Escherichia coli drug resistance isolated from different parts of
Tibet yak in order to provide scientific evidence for controling
Escherichia coli.
PCR analysis of
drug resistance phenotype and
drug resistance gene of
aminoglycosides,florfenicol and
sulfonamides were carried out in 200
strains of
yak-derived
Escherichia coli from 6 different places in
Tibet.The results showed that the resistance rate of
aminoglycoside to
yak-derived
Escherichia coli was 96% of
amikacin,94.59% of
streptomycin,19% of
neomycin,23% of
gentamicin,19% of
kanamycin.The
detection rate of rmtB
gene was 100%.The
drug resistance rate of florfenicol was 25% with 25%
detection rate of resistance geneflor.The resistance rate of
sulfonamides was 32% with 7%,7% and 17%
detection rates of sul1,sul2 and sul3 respectively
genes,Moreover,the result of
drug resistance phenotype was consistent with that of
drug resistance gene.Meanwhile,the expression of sull
gene in different regions of
Tibet were different in
drug resistance of the bacilli,and those from the
population-intensive and flow-sensitive Lhasa,Nyingchi and Xigaze were more resistant to
drug resistance,while those from Ali,Shannan and Nagqu were relatively
light.The above-mentioned
drug-resistant
phenotype and
drug resistance genes were detected in different cities and regions of
Tibet,and there were multiple
drug-resistant phenomena.The results showed that there was resistance to
Escherichia coli in Tibetan
yak,which should be paid
attention to,and suggested that the in Tibetan area the
antibiotics should be rationally used to reduce the
drug resistance.