<p><b>OBJECTIVE</b>To investigate the effects of
arsenic trioxide (AsO) on Cdc20 and Mad2 in process of AML
HL-60 cell proliferation.</p><p><b>
METHODS</b>The proliferation of
HL-60 cells was detected by
CCK-8 method at different concentrations of
arsenic trioxide for 24, 48 and 72 hours. The
cell morphological changes were observed by inverted
microscopy. The expressions of Mad2 and Cdc20
mRNA and
protein in
HL-60 cells treated with AsO for 48 h were detected by
real-time PCR and
Western blot respectively.</p><p><b>RESULTS</b>
Arsenic trioxide significantly inhibited the
HL-60 cell proliferation and displayed a good
time-
dose correlation. RT-PCR and
Western blot showed that the expression of Mad2 was up-regulated and the expression of Cdc20 was down-regulated in
HL-60 cells treated with
arsenic trioxide of different concentration (4,8,10 µmol/L).</p><p><b>CONCLUSION</b>
Arsenic trioxide can inhibit the
human acute myeloid leukemia HL-60 cell proliferation, and its mechanism may be related with
up-regulation of Mad2 expression and
down-regulation of Cdc20 expression.</p>