Effect of Miscanthus sinensis var. purpurascens Flower Extract on Proliferation and Molecular Regulation in Human Dermal Papilla Cells and Stressed C57BL/6 Mice / 中国结合医学杂志
Gi-Hee JEONG; William-A BOISVERT; Mei-Zhu XI; Yi-Lin ZHANG; Young-Bin CHOI; Sunghun CHO; Sanghyun LEE; Changsun CHOI; Bog-Hieu LEE; Gi-Hee JEONG; William-A BOISVERT; Mei-Zhu XI; Yi-Lin ZHANG; Young-Bin CHOI; Sunghun CHO; Sanghyun LEE; Changsun CHOI; Bog-Hieu LEE.
Chinese journal of integrative medicine
; (12): 591-599, 2018.
Artículo
en Inglés
| WPRIM | ID: wpr-691378
<p><b>OBJECTIVES</b>To investigate the hair growth-promoting effect of Miscanthus sinensis var. purpurascens (MSP) flower extracton on in vitro and in vivo models.</p><p><b>METHODS</b>MSP flower extract was extracted in 99.9% methanol and applied to examine the proliferation of human dermal papilla cells (hDPCs) in vitro at the dose of 3.92-62.50 μg/mL and hair growth of C57BL/6 mice in vivo at the dose of 1000 μg/mL. The expression of transforming growth factor β1 (TGF-β1), hepatocyte growth factor (HGF), β-catenin, substance P was measured by relative quantitative realtime polymerase chain reaction. Histopathological and immunohistochemical analysis were performed.</p><p><b>RESULTS</b>MSP (7.81 μg/mL) down-regulated TGF-β1 and up-regulated HGF and β-catenin in hDPCs (P<0.01). MSP (1000 μg/mL)-treated mice showed the earlier transition of hair follicles from the telogen to the anagen phase. The number of mast cells was lower in the MSP-treated mice than in other groups (P<0.05 vs. NCS group). Substance P and TGF-β1 were expressed in hair follicles and skin of the MSP group lower than that in negative control. Stem cell factor in hair follicles was up-regulated in the MSP-treated mice (P<0.01).</p><p><b>CONCLUSIONS</b>The MSP flower extract may have hair growth-promotion activities.</p>
Asunto(s)
Animales Femenino Humanos Antioxidantes Farmacología Recuento de Células Proliferación Celular Quinasas MAP Reguladas por Señal Extracelular Metabolismo Flores Química Folículo Piloso Biología Celular Factor de Crecimiento de Hepatocito Metabolismo Mastocitos Biología Celular Ratones Endogámicos C57BL Fosforilación Extractos Vegetales Farmacología Poaceae Química ARN Mensajero Genética Metabolismo Piel Metabolismo Factor de Células Madre Metabolismo Estrés Psicológico Patología Sustancia P Metabolismo Factor de Crecimiento Transformador beta Genética Metabolismo Factor A de Crecimiento Endotelial Vascular Genética Metabolismo beta Catenina Metabolismo
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