Objective To investigate the difference between
mammary gland tissues and
breast cancer tissues.
Methods Monoclonal antibodies against Mam-A immunized
epitopes were screened for immunohistochemical
staining of normal
breast tissues and
breast cancer tissues.The average optical density was used as an index to identify the quantitative data by
computer-aided
technology to screen
epitope-specific
antibodies with significant difference in
staining characteristics between two types of
tissues.Furthermore the feasibility and
effectiveness of
breast cancer diagnosis were evaluated.Results Four anti-Mam-A
epitope-specific
monoclonal antibodies,mAb1152,mAb11617,mAb995 and mAb656,were obtained.Immunohistochemical
staining showed that the average density of mAb1152,mAb11617 and mAb995 was significantly different between the two types of
tissues.The difference was significant between normal
breast tissues and
breast cancer tissues under the same conditions.The results showed that mAb11617 was better than mAb1152 and mAb995.At the best working point,mAb11617 was the best,the
specificity was 90% and the
sensitivity was 59.62%.Further
analysis showed that the
sensitivity of mAb11617 combined with mAb995 in the
diagnosis of in situ
breast cancer was 81.48% and the
specificity was 90%,which was of great diagnostic significance.Conclusion There is significant difference between
breast tissues and
breast cancer tissues in Mam-A
protein immunological activity or expression.This difference,which can be recognized by the specific antibody
staining and
computer aided
technology,is of important diagnostic value.