Purpose To investigate the expression of globular C1q receptor (gC1qR) in
ovarian cancer and to explore its potential molecular mechanism.
Methods Retrospective
analysis was made on 48
ovarian cancer tissues and
ovarian cancer cell line SKOV3.
Real time PCR and
Western blot analysis were applied to detect the levels of gC1qR
mRNA and gC1qR
protein expression.The
abilities of SKOV3
cells proliferation activity and quantity,migration and
apoptosis were respectively assessed by M'TT,transwell assay and
flow cytometry.Besides,the intracellular ROS was estimated via the
fluorescence of H2DCFDA,the
mitochondrial membrane potential was tested using a JC-1 probe,and the intracellular Ca2+ was assessed via Fluo-3/AM.Results The expressions of gC1qR
gene was obviously decreased in the group of
ovarian cancer tissues when compared with normal ovarian
tissues group (2.61 ±0.34 vs 7.32 ± 1.25,0.20 ± 0.02 vs 0.67-± 0.06,P < 0.001).Overexpression of gC1qR
gene could result in significant
up-regulation of
ovarian cancer cell apoptosis and
down-regulation in proliferation and migration,and showed significant
cell apoptosis morphology.Simultaneously,the intracellular ROS and Ca2+ were obviously increased,and the
mitochondrial membrane potential was obviously decreased.Conclusion gC1qR
gene may
play an important
role in the
apoptosis of
ovarian cancer cells.In this process,gC1qR
gene can induce
apoptosis of
ovarian cancer cells by inducing
mitochondrial dysfunction.