Objective To explore the effect of
ginsenoside Rg1 on
leukemia stem cells through comparing the
biological senescence characteristics of HSCs in the
patients with
leukemia and healthy people,and provide new ideas and
methods for
leukemia prevention and
treatment.
Methods Fifteen cases of normal
bone marrow in normal group and sixteen cases of
chronic myeloid leukemia in
leukemia group were divided into
control group and Rg1 group,respectively.The
control group used the conventional
culture.The Rg1 group used the
culture system with 10 μg/mL
ginsenoside Rg1,other conditions were the same as
control group.The
bone marrow mononuclear
cell of all groups were extracted after 2 days,and the CD34 +/CD38-
cells population was isolated and purified by immunomagnetic
adsorption cell sorting(MACS).The purity of the
cells and
cell cycles phase were detected by
flow cytometry.
Cell viability was detected by
trypan blue staining.The percentage of positive
cells was detected by SA-β-gal
staining.
CCK-8 detected the CD34 +/CD38-proliferation
ability of each group.Results The ratio of CD34 +/CD38-
cell population was (1.76 ± 0.34) % in every 1 × 106 BMNCs before sorting;the proportion of CD34 +/CD38-
cell population per 1 × 106
cells after immunomagnetic sorting was (91.15 ± 2.41)%.The positive rate of SA-β-gal
staining in
human bone marrow CD34 +/CD38-
cells of
leukemia Rg1 group was significantly higher than that in
leukemia control group,the difference was not significant (P > 0.05);meanwhile there was no significant difference between normal
control group and normal Rg1 group,but higher than that in
leukemia control group,the difference was significant(P < 0.05).
CCK-8 results showed that the proliferation of CD34 +/CD38-
cells was significantly increased in
leukemia control group than those in the other groups.The
survival rate of CD34 +/CD38-
cells in
human bone marrow was 99.1% in all groups.
Cell cycle phase results showed that the G1 arrest of CD34 +/CD38-
cells in
leukemia control group was significantly lower than those in the other three groups.Conclusion CD34 +/CD38-
cells in
chronic myeloid leukemia patients may be caused by some
chronic myeloid leukemia.
Ginsenoside Rg1 can effectively delay the process of
aging.