Aim To investigate the effect of
liver X receptor (LXR) activation on the proliferation of hippocampal
neural stem cells in global
cerebral ischemia /
reperfusion (I/R)
mice ,and its mechanisms.
Methods A total of 75 C57BL/6
mice were randomly divided into three groups,namely the sham operation group,the cerebral I/R group and the cerebral I/R with TO901317
treatment (I/R + TO90) group.The I/R
mouse model was induced via the bilateral
common carotid artery occlusion.HE
staining was used to detect the pathological changes in
hippocampal CA1 region .
Immunohistochemistry was executed to detect
hippocampus DCX +
cells .
Immunofluorescence of
BrdU was implemented to detect the proliferation
neural stem cell .
Morris water maze test was used to assess
spatial learning and
memory in
mice .
Western blot was used to detect the expression of
hippocampus LXRα,LXRβ,ABCA1,p-ERK1/2,t-ERK1/2,p-CREB,t-CREB,
BDNF .Results LXR activation improved cognitive recovery(P <0.01),and induced the proliferation of
neural stem cells (P < 0.01) in I/R
mice .The expressions of hippocampal ABCA1,p-ERK1/2,p-CREB,
BDNF in I/R + TO90 group
mice also increased (P < 0.01).Conclusions LXR activation can induce the proliferation of hippocampal
neural stem cells and facilitate cognitive recovery following global cerebral I/R in
mice ,which may be related to the activation of hippocampal ERK1/2-CREB-
BDNF pathway and then promoting endogenous
neurogenesis in the
hippocampus DG region of I/R
mice .