A splanchic
artery occlusion for 90 min followed by
reperfusion of the
mesenteric circulation resulted in a severe form of circulatory
shock characterized by endothelial dysfunction, severe
hypotension, marked intestinal
tissue injury, and a high mortality rate. The effect of
rutin, a
flavonoid having antiprostanoid, anti-inflammatory, antithrombotic,
antioxidant effect, were investigated in a model of splanchnic
artery occlusion (SAO)
shock in
urethane anesthetized
rats. Occlusion of the
superior mesenteric artery for 90 min produced a severe
shock state resulted in a
fatal outcome within 120 min of
reperfusion in many
rats.
Rutin was given as a bolus (1.28 mg/kg) 10 min prior to
reperfusion.
Administration of
rutin significantly improved mean
arterial blood pressure in comparison to vehicle treated
rats (p<0.05).
Rutin treatment also resulted in a significant attenuation in the increase in
plasma amino
nitrogen concentration, intestinal
myeloperoxidase activity, intestinal
lipid peroxidation, infiltration of
neutrophils in
intestine and
thrombin induced adherence of
neutrophils to superior mesentric
artery segments. These results suggest that
rutin provides beneficial effects in part by preserving endothelial function and attenuating
neutrophil accumulation in the ischemic reperfused
splanchnic circulation.