@#Objective To establish a
patient-derived
tumor xenograft (PDTX) model and to observe the latency and rate of
tumor formation,
tumor size,
tumor invasion and
metastasis of transplanted
tumors.
Methods Seven
patients with
chest tumor in Drum Tower
Hospital from April to December 2015 were chosen. There were 5
males and 2
females with age ranging from 61-71 years, including 4
patients of esophageal
tumor and 3
patients of
lung tumor. PDTX model was established by surgical removal of fresh
tumor tissues of these
patients and
transplantation in NODPrkdcem26Il2rgem26Nju subcutaneous (NCG)
mice. The latency and rate of
tumor formation,
tumor size,
tumor invasion and
metastasis of transplanted
tumors were observed, and
pathology of HE
staining and immunohistochemical testing results were compared between PDTX model and the
patients. Results PDTX model was successfully established in 4
patients, and the success rate was 66.7%, including 2
patients of
esophageal cancer. The PDTX model retained the differentiation, morphological and structural characteristics of original
tumors. Conclusion
Pathology and
molecular biology characteristics of PDTX model are consistent with the original
tumor, which can be an “
avatar” of
tumor patients for clinical pharmacodynamics
screening and new
drug research and development.