Objective The
bioinformatics software and database were involved in prediction of the target
genes of hsa-miR-26a,so as to lay
foundation and provide theoretical basis for the further studies of hsa-miR-26a
biological function in fetal
lung development.
Methods All
literature of miR-26a were searched in
PubMed and Google ; miRBase database was used to obtain the sequence of hsa-miR-26a and to analyze its conservation.The target
genes of miR-26a were predicted using
miRNA target
gene database miRGen2.0.TargetScans and PicTar were used to predict target
genes of hsa-miR-26a.The intersection of the 2 results and validated targets from DIANA LAB-TarBase 6.0 datebase was analyzed by
gene ontology and pathway
analysis.Results Hsa-miR-26a had been reported in
cancer,
cell differentiation,organ development,the
immune system as well as involved in other
biological process;hsa-miR-26a was highly conservative among different species.The functions of these target
genes were enriched in translation
regulation,the
protein modification,the
regulation of celluar proliferation,
apoptosis and differentiation process,
kinase activity
regulation,target
genes exist in all of the
cell components,including
cell membrane,
cytoplasm and nucleus.The
Wnt signaling pathway,
mitogen-activated protein kinase signaling pathway,
transforming growth factor-β signaling pathway,the pathway of
tumor p53 signaling pathways,
cell cycle and
adherens junction pathways were significantly enriched and
prostate cancer,
small cell lung cancer,
colorectal cancer,
renal cell cancer and
glioma were mainly involved (all P < 0.05).Conclusions The target
genes of hsa-miR-26a are enriched in multiple
biological process.The prediction and
bioinformatic analysis of miR-26a target
genes lay the
foundation for the further study in fetal
lung development.