Objective To explore the effect of vaginal micro-
environment alterations and HPV1 6
infection and their interaction in the progression of
cervical intraepithelial neoplasia.
Methods The participants of this study came from the cervical lesions study cohort in Shanxi province,including 623
women with normal cervical (NC),303
patients with pathogenically diagnosed low-grade
cervical intraepithelial neoplasia (CIN Ⅰ) and 93
patients with pathogenieally diagnosed high-grade
cervical intraepithelial neoplasia (CIN Ⅱ/Ⅲ).The data of the demographic characteristics of the study subjects and factors related to
cervical intraepithelial neoplasia were collected,and
HPV16 infection were detected by using flow-through hybridization
technology and H2O2,β-
glucuronidase,clotting
enzyme,
neuraminidase and leucocyte
esterase in vaginal
secretions were detected by using the combined
detection kit of aerobic
vaginitis and
bacterial vaginosis.
pH value and vaginal cleanliness were also detected at the same
time.The database was established and analyzed by SPSS statistical
software (version 22.0).Results The
HPV16 infection rate (trend x2=55.45,P<0.001) and the abnormal rates of H2O2 (trend x2 =26.19,P<0.001),
pH (trend x2=5.06,P=0.024),vaginal cleanliness (trend x2=19.55,P<0.001),β-
glucuronidase (trend x2 =17.52,P<0.001) and
neuraminidase (trend x2 =14.90,P< 0.001) increased gradually along with the severity of
cervical intraepithelial neoplasia,but the abnormal rates of clotting
enzyme and leucocyte
esterase showed no same trend.The results of GMDR model
analysis showed that there was interaction between
HPV16 infection and
abnormalities of H2O2,β-
glucuronidase,clotting
enzyme and
neuraminidase in CIN Ⅰ group,and the interaction between
HPV16 infection and the
abnormalities of vaginal cleanliness,H2O2,β-
glucuronidase and neumminidase in CIN Ⅱ/Ⅲ group.Conclusion Our findings indicated that the vaginal micro-
environment alterations and HPV1 6
infection could increase the
risk of
cervical intraepithelial neoplasia,and they might have an important synergistic effect in the progression of
cervical intraepithelial neoplasia.