Objective To deduce the normal range of thyroglobulin ( Tg ) of adults senum in Liaoning Province . Methods From February to November 2017 , in Kangping County of Shenyang ( inland group ) and Gaizhou City of Yingkou (coastal group), the Tg levels of adults in different age groups were investigated according to the cluster stratified samplingmethod. Adults were divided into young (18 - 44 years old), middle (45 - 59 years old ) , and elderly (≥60 years old ) groups , and fastingblood samples were collected . Levels of Tg , thyroid function [ thyroid stimulating hormone (TSH), free triiodothylaminogenic acid (FT3), free thyroxine ( FT4 ) ] , thyroglobulin antibody (TgAb) and thyroid peroxidase antibody (TPOAb) were determined by automatic electrochemiluminescence immunoassay analyzer. Statistical analysis was performed on Tg measured values of normal thyroid function and antibody-negative adults, and the percentile range (P2.5 - P97.5) was used to calculate the 95% normal range of both sides. Results A total of 685 adults were included, with a Tg level of 9.86 μg/L, including 333 adults in the inland group with a Tg level of 8.97 μg/L, and 352 adults in the coastal group with a Tg level of 10.44 μg/L. There were 255 males and 430 females, Tg levels were 9.39 and 9.99 μg/L, respectively. There were 179, 307 and 199 adults in the young, middle and elderly groups, Tg levels were 8.84, 9.22, and 11.73 μg/L, respectively, the differences were statistically significant (H=9.607, P<0.05). The normal range of Tg in adults was 1.23-58.63μg/L, and the normal ranges of Tg in the young, middle and elderly groups were 1.62 - 41.90, 0.99 -54.58, and 0.94 - 85.78 μg/L, respectively. Conclusion Successful establishment of the normal range of adultsserum Tg in Liaoning Province.