Objective To investigate the expression of
proline rich tyrosine kinase 2 (Pyk2) in
non-muscle invasive bladder cancer,and analyze its correlation to clinicopathologic features and
prognosis of
non-muscle invasive bladder cancer.
Methods 114 surgical specimens and 50 normal
bladder mucosa specimens were collected from 114
non-muscle invasive bladder cancer patients who underwent TURBT at our
hospital,from June 2013 to March 2018.Of the 114
patients,63 were
male and 51 were
female,
aged 42-87 years,average age of (63.6 ± 13.8) years,73 cases of
tumor <3 cm,41 cases of
tumor ≥3 cm,83 cases were single and 31 cases were multiple
tumor,53 cases were high grade and 61 cases were low grade,59 cases were Ta and 55 cases were T1 stage.Pyk2
protein expression was detected by
immunohistochemistry and
western blot.The correlation of the expression of Pyk2 with clinicopathologic features,including
gender,age,
tumor size,the number of
tumors,histological grade and clinical stage were analyzed.
Survival analysis was calculated by using the Kaplan-Meier
method,and the difference in
survival curve was analyzed by using the log-rank test.
Association of Pyk2 expression with
prognosis of
non-muscle invasive bladder cancer analyzed by using the Cox proportional
hazards regression model.Results Compared with normal
bladder tissues,expression of Pyk2
protein was increased in
bladder cancer tissue significantly(0.571 ±0.230 vs.0.253 ± 0.152,P <0.01).The expression of Pyk2
protein was closely related to clinical stage(P =0.027) and grade(P =0.010),rather than
gender (P =0.275),age (P =0.419),
tumor size (P =0.317),and
tumor number(P =0.208).The
recurrence rate in the Pyk2 positive group and negative group were 46.1% (35/76)and 28.9% (11/38)respectively.The progression rate in the Pyk2 positive group and negative group were 35.5% (27/76) and 10.5 % (4/38) respectively.
Survival analysis suggested expression of Pyk2 in
non-muscle invasive bladder cancer had a significant relation to
recurrence-free
survival rate(P <0.001) and
progression-free survival rate(P =0.003).In the multivariable Cox
analysis,we found that Pyk2
protein was an independent predictor of
recurrence-free
survival rate(HR 0.245,95% CI 0.078-0.768,P =0.016) and
progression-free survival rate (HR 0.095,95% CI 0.012-0.764,P =0.027).Conclusions The expression of Pyk2 in
non-muscle invasive bladder cancer was significantly increased.The expression of Pyk2 has a significant relation to
recurrence and progression of
non-muscle invasive bladder cancer.High Pyk2 expression is an independent
prognostic factor in
non-muscle invasive bladder cancer.