To clone successfully A1A2 and A3C2 gen fragment encoding factor VIII. Subject and
Method:
Amplify A1A2 and A3C2 gene fragments by PCR from humancDNA. PCR products were ligated into cloning vector pQE \u2013 30UA. Recombinant plasmids were transformed into E.coli DH5 alpha host strain. Inserted A1A2 and A3C2 gene fragments were checked by PCR and restriction enzymes.
Result:
Successfully amplifying functional gene fragments encoding factor VIII using specific primers.
Conclusion:
Obtaining pQE \u2013 30UA vector carrying A1A2 and A3C2 fragments encoding factor VIII. This is the premise result for the next studies on synthesis of recombinant factor WIII and application of genetic therapy.