PURPOSE: Activated leukocyte cell adhesion molecule (
ALCAM ), a member of the
immunoglobulin superfamily, is highly expressed on
dendritic cells .
ALCAM and its receptor CD6 are co-stimulatory molecules in the
immunological synapse ; their interaction is required for
T cell activation. While
atopic dermatitis (AD) is recognized as a T helper 2 (Th2)-mediated allergic
disease , the
role of
ALCAM in its pathogenesis is unclear.
METHODS: ALCAM levels were measured in the
serum of AD
patients and AD-induced murine model by
ovalbumin treatment . We next investigated transepidermal
water loss , clinical score, Th2-
immune responses ,
skin barrier gene expression and
T-cell activation using wild-type (WT) and
ALCAM deficiency mice . An
oxazolone -induced AD-like model was also established and analyzed using WT- and
ALCAM -deficient
mice .
RESULTS: We found that
serum ALCAM levels were elevated in pediatric AD
patients as well as WT AD
mice , whereas Th2-type
cytokine production and AD symptoms were suppressed in
ALCAM -deficient
mice . In addition, CD4+ effector
T-cell counts in murine
skin and
skin -draining
lymph nodes were lower in
ALCAM -deficient
mice than in their WT counterparts.
ALCAM deficiency was also linked to higher expression of
skin barrier genes and number of
lamellar bodies .
CONCLUSIONS: These findings indicate that
ALCAM may contribute to AD pathogenesis by meditating a Th2-dominant
immune response and disrupting the
barrier function of the
skin .