Recharacterization of the Canine Adenovirus Type 1 Vaccine Strain based on the Biological and Molecular Properties
Dong-Kun YANG; Ha-Hyun KIM; Eun-Jin LEE; Jae-Young YOO; Soon-Seek YOON; Jungwon PARK; Chae-Hyun KIM; Ho-Ryoung KIM.
Journal of Bacteriology and Virology
; : 124-132, 2019.
Artículo
en Inglés
| WPRIM | ID: wpr-764239
Canine adenovirus type 1 (CAV-1) infection results in hepatitis in dogs. In this study, we investigated the biologic and genetic characteristics of the CAV-1 vaccine strain (CAV1V) to improve quality control about CAV vaccine. The identity of CAV1V as CAV-1 was confirmed based on its cytopathic effects and the results of hemagglutination (HA) and immunofluorescence assays, and electron microscopy. The CAV1V strain reached 10(7.5) TCID(50)/mL in MDCK cells at 4 days post-inoculation and exhibited hemmagglutination activity of 256 U using guinea pig erythrocytes. Intranuclear fluorescence in the infected cells was observed and typical adenoviruses were observed in electon microscope. CAV1V strain was identified as a CAV-1 strain by nucleotide sequence analysis. In a comparison of the nucleotide sequences of the fiber genes of several CAV strains, CAV1V showed the highest similarity (99.8%) with the GLAXO strain, which was isolated in Canada. Our biological characterization of CAV1V will facilitate quality control of the canine hepatitis vaccine.
Biblioteca responsable:
WPRO
Texto completo
Documentos relacionados