OBJECTIVE@#To investigate the effects of
Listeria monocytogenes infection on hematopoietic
stem and
progenitor cell (HSPC) composition,
cell cycle and
cell colony-forming
ability in
mouse bone marrow.@*
METHODS@#The C57BL/6J
mice were divided into infected group and
control group. The
mice in injected group were infected intraperitoneally with 6.7×10 CFU
Listeria monocytogenes,while the
mice in
control group were injecfed with PBS of same volume.The
serum levels of IFNγ were detected at different
time points. After 24 hours, the HS/PC composition,
cell cycle and
cell colony-forming
ability in
bone marrow of
mice were measured, and the difference between the
control group and the infected group was statistically analyzed.@*RESULTS@#
Serum IFNγ levels peaked at 24 hours after
infection with
Listeria monocytogenes. After 24 h, the proportion of LSK, LSK in
S phase, and short-term
hematopoietic stem cells (ST-HSC) in the infected group were significantly higher than those in the
control group (P<0.001), long-term
hematopoietic stem cells (LT-HSC) and the proportion of LT-HSC in
S phase were significantly increased (P<0.01), and the
cell colony-forming
ability of
bone marrow significantly decreased (P<0.01). [WTHZ]
Conclusion:
[WTB1]After
infection with
Listeria monocytogenes,
bone marrow hematopoietic stem cells enter the proliferative
state from
rest, the
cell colony-forming
ability decreases, suggesting that
Listeria monocytogenes infection can cause
hematopoietic stem cell depletion.