In the present study,non-targeted
metabolomics technique was used to screen potentially susceptibility
biomarkers in
patients with mild
liver function
abnormalities during long-term use of
Chinese herbal compound. According to the inclusion and exclusion criteria,we collected 7 cases of
patients with abnormal
liver function during the period of complete taking
Chinese herbal medicine( 60 days),and 18 cases of
patients with normal
liver function in re-examination from the
reproductive medicine center in our
hospital.
Ultra performance liquid chromatography coupled with
time-of-flight
mass spectrometry(
UPLC-Q-TOF/MS~E)
technique combined with Progenesis
QI software was used to analyze the differential
biomarkers in
serum of
patients with wild
liver function
abnormalities and normal
liver function. 11 potential
biomarkers such as
bilirubin,
pantothenic acid,hippuric
acid,
sphingomyelin,
palmitic acid,and
oleic acid were tentatively identified. Metabolic disorders in
patients with
herbal-induced mild
liver abnormality were mainly related to two pathways
pantothenic acid and
coenzyme A biosynthesis and
linoleic acid metabolism. It could provide a reference for the
early warning of mild
liver function
abnormalities of
patients that may be caused by long-term use of
Chinese medicine compound in clinical application,and
will lay a
foundation for further
understanding the endogenous substance changes in different levels of
liver injury.