The study is aimed to test the effect of
Huangqin Tang (HQT) on
serum metabolic profile in
rats with
ulcerative colitis, and explore its possible action mechanism for
ulcerative colitis (UC)
rats. The model of UC
rats with
cell immunoreactivity was made using a compound
method (trinitrobenzene
sulfonic acid plus
ethanol).
Rats were randomly divided into the
control group, the model group, and HQT group.
Ultra performance liquid chromatography tandem mass spectrometry (UHPLC-MS),
principal component analysis (
PCA) and partial
least squares-
discriminant analysis (PLS-DA) were employed to analyze the
metabolic profile among normal group, the model group, HQT group. Potential
biomarkers were screened in the
serum based on the variable importance
projection (VIP) value > 1, P< 0.05. As compared with the normal group, 16 potential
biomarkers such as
valine,
tryptophan,
lactic acid and
urea were found and identified in the
serum of model group
rats. As compared with the model group, a part of the
biomarkers were restored nearly to a normal
state after HQT
administration for 10 days.
Metabolomic analysis revealed that the HQT has a certain
therapeutic effect in UC
rats, and the mechanism may be related to
regulation of
lipid metabolism,
amino acid metabolism and
energy metabolism.