Study on the technology for extracting lignans in schisand ra chinensis (Turcz ) Baill by AB-8 macroporous resin / 药学实践杂志
Yiping JIANG; Liming XUE; Hailiang XIN; Qiaoyan ZHANG; Luping QIN.
Journal of Pharmaceutical Practice
; (6): 520-525, 2017.
Artículo
en Zh
| WPRIM | ID: wpr-790809
Objective To study the conditions for isolating and purifying lignans in schisandra chinensis (Turcz) Baill by AB-8 type of macroporous adsorption resin .Methods The content of Schizandrol ,Deoxyschizandrin ,r-Schisandrin was deter-mined by HPLC .The optimum separation and purification process of lignans from schisandra chinensis (Turcz) Baill with AB-8 macroporous adsorption resin were identified by static and dynamic adsorption ,desorption tests .The sample volume ,concen-tration ,eluting velocity ,and ethanol concentration were investigated .Results The optimum conditions for isolating and purif-ying lignans in schisandra chinensis (Turcz) Baill by AB-8 macroporous adsorption resin were as followsdosage of sample liq-uid was 1BV ,concentration of sample solution was 9 .159-16 .523 mg/ml ,ratio of diameter to height of resin column 15 ,ad-soption flow rate was 2 .5 BV/h ,eluted by 5 BV 30％ ethanol ,followed by 10BV 95％ ethanol .The transfer rate of lignans was 77 .07％ with 22 .06％ of total lignan content .Conclusion AB-8 macroporous adsorptive resin can effectively isolate and purify lignans from schisandra chinensis (Turcz) Baill .This low cost process is easy to operate and with high industrial pro-duction value .
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WPRO
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