Objective To undersyand the genetic characteristics of
Enterovirus Type 71 (EV 71 )isolated from clinical specimens of
children with
hand-
foot-
mouth disease (HFMD)in Shaoxing city from 2012 to 2013.
Methods RNA was extracted from specimens collected from stool,
throat swab and vesicle from
children with HFMD,and EV 71 were identified by
reverse transcriptase polymerase chain reaction (RT-PCR).Ten positive samples were isolated and were amplified by segmented
PCR,then,they were spliced into a full-length after sequenced according to the reference
strains. Homology
analysis on region VP1 and
phylogenetic tree were carried out based on
nucleotide sequence and
amino acid sequence.Results The results indicated that the ten isolated
strains were all EV 71 positive.
Sequence alignment of VP1 showed that the
nucleotide and
amino acid homogeneity of these 10
strains were related to C4a
genotypes,with 95.06%-97.87% and 93.60%-98.99% respectively.
Phylogenetic tree based on VP1 indicated that these 10
strains all belonged to C4a
genotype.Conclusion The identified EV 71
strains isolated from HFMD
children in Shaoxing city belong to
genotype C4a.