HCCLM3 cells were added into low, medium or high-concentration groups of glucose. It was found in cell counting kit-8(CCK-8)that the high concentration of glucose had the most obvious effect on HCCLM3 cells proliferation. Based on the above experimental result, HCCLM3 cells treated with high concentration of glucose was selected and then different concentrations of berberine (5, 10, 20, 30, 40, 50 μmol·L-1) was added for in vitro intervention for 24 h. Then the effect of each drug group on the proliferation of HCCLM3 cells were studied. At the same time, the control group of metformin was arranged. After that, the changes of apoptosis rate were observed by flow cytometry, and the expression levels of B-cell lymphoma-2(Bcl-2)and autophagygenes Atg5, Beclin1 were detced by Real-time polymerase chain reaction (Real-time PCR).
Result:
With the increase of glucose concentration, HCCLM3 cell had the strongest migration and proliferation ability in high glucose group (P-1), and the inhibition rate was 33.86%, 40.75% (PPPP P