The sixty mice were randomly divided into normal group, model group,bicyclol group (62.5 mg·kg-1), and low, medium and high-dose Fuzheng Quxie prescription groups(50.3,67.0,83.8 g·kg-1). The normal group and the model group were given the equal volume of pure water for 7 d. They were fasted for 12 hours before the last administration. At 2nd hour after the last administration, phosphatebuffer(PBS) was injected into the caudal vein of the normal group, and ConA (20 mg·kg-1) was injected into the caudal vein of the other groups for modeling. The animals were put to death six hours later after the injection, and the expressions of TNF-α, Fas, FasL and CD68 in livertissues were observed by immunohistochemistry.
Result:
Compared with normal group, the expressions of TNF-α, Fas, FasL and CD68 in the livertissues of the model group were significantly increased(Pα, FasL and CD68 in livertissues of the bicyclol group were significantly decreased compared with the model group(Pα, FasL and CD68 in livertissues were significantly decreased in medium and high-dose Fuzheng Quxie prescription groups (PP