Objective: To observe the protective effect and mechanism of combination of puerarin combined with tanshinone ⅡA on
diabetes mellitus (DM)
rats with vascular lesions.
Method: The SD
rats (fed with
high-fat diet ) were administrated with
streptozotocin (STZ) through
intravenous injection to make the model of diabetic vascular lesions. The successfully modeled
rats were randomly divided into the model
control group , the high-
dose group (0.5 g·kg-1+1.0 g·kg-1), the middle-
dose group (0.25 g·kg-1+0.5 g·kg-1), the low-
dose group (0.05 g·kg-1+0.1 g·kg-1), the puerarin group (0.25 g·kg-1), the tanshinone ⅡA group (0.5 g·kg-1) and the positive
control group (
Metformin , 0.09 g·kg-1). Each group was administrated with
drugs respectively by gavage for 70 days. After intervention in each group, the general conditions and
body weight of the
rats were observed. The contents of
blood grucose and
blood lipids were determined by automatic biochemical analyzer. The contents of
insulin ,
advanced glycation end products (AGEs),
superoxide dismutase (SOD),
glutathione peroxidase (GSH-Px) in
serum , the contents of
tissue plasminogen activator (t-PA),
plasminogen activator inhibitor -1 (
PAI-1 ) and
thromboxane B2 (TXB2) in
plasma , as well as the contents of AGEs and oxidized
low-density lipoprotein (ox-LDL) in
aorta homogenate were detected by
enzyme-linked immunosorbent assay (
ELISA ). The content of
malondialdehyde (MDA) in
serum was determined by chemical
colorimetry . Pathological changes of coronary
tissue were observed by htoxylin
eosin (HE)
staining . The expression of
PAI-1 protein of
aorta was observed by
immunohistochemistry .
Result: Compared with the normal
control group , in the model group, the levels of
blood grucose and
blood lipids (PPPP2 in
plasma (PPPPPPPPP2 in
plasma (PPPPPPP
Conclusion: Puerarin combined with Tanshinone ⅡA could relieve vascular lesions of DM
rats . The mechanisms may be related to the reduction of
oxidative stress and the
regulation of coagulation-
fibrinolysis system.